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Objective:To retrospectively analyze the differences of survival, recurrence, acute side effects and prognostic factors between early stage (stage ⅠB-ⅡA) cervical cancer patients with intermediate risk factors receiving postoperative concurrent chemoradiotherapy (CCRT) or radiotherapy (RT) alone.Methods:Clinical data of 211 patients with intermediate risk factors after early stage cervical cancer surgery admitted to Department of Radiation Oncology of General Hospital of Ningxia Medical University from January 2016 to December 2018, were retrospectively analyzed. Among them, 91 cases were assigned in the RT group and 120 cases in CCRT group. The 3- and 5-year overall survival (OS), progression-free survival (PFS), recurrence and acute side effects were compared between two groups by Chi-square test. Univariate analysis of OS and PFS was performed by Kaplan-Meier method and log-rank test was performed. Multivariate prognostic analysis was conducted by using Cox model. Results:The 3- and 5-year OS of 211 patients were 95.0% and 93.8%, respectively. The 3- and 5-year PFS were 86.8% and 83.2%, respectively. The OS of CCRT and RT group were 93.9%, 96.5% (3-year), 91.8%, 96.5% (5-year) respectively ( χ2=1.763, P=0.184), and the PFS were 84.4%, 89.9% (3-year), 79.3%, 88.3% (5-year) ( χ2=2.619 ,P=0.106), with no difference between the two groups. The total recurrence rate was 15.64%, and there was no significant difference in the recurrence rate and recurrence area between two groups ( χ2=2.623 ,P=0.105; χ2=6.745 ,P=0.080). Locoregional recurrence and lung metastasis were the main patterns of failure. Multivariate prognostic analysis showed that pathological type might significantly affect the OS ( χ2=3.849, P=0.05), and depth of invasion significantly affected the PFS ( χ2=4.095, P=0.043). The incidence of acute gastrointestinal side effect and bone marrow suppression in the CCRT group was significantly higher than that in the RT group ( χ2=56.425, 27.833; both P<0.001). Conclusions:Patients with intermediate risk factors after early cervical cancer surgery obtain high efficacy after radiotherapy. The main patterns of failure are locoregional recurrence and lung metastasis. The pathological type may be an independent prognostic factor of OS and the depth of invasion is an independent prognostic factor of PFS. Compared with RT, CCRT increases the risk of acute gastrointestinal side effects and myelosuppression, which can be tolerated. There is no significant difference in the clinical efficacy between RT and CCRT, which remains to be validated by large sample size studies.
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Objective:To retrospectively analyze the prognosis and related risk factors of lymph node metastasis in early-stage (Ⅰ B-Ⅱ A) cervical cancer patients with postoperative positive lymph nodes who were treated with intensity modulated radiotherapy (IMRT). Methods:A retrospective analysis was conducted for 292 early-stage cervical cancer patients with postoperative high and/or moderate risk factors who were treated with pelvic-abdominal IMRT with/without concurrent chemotherapy in Ningxia Medical University General Hospital from January 2016 to December 2018. These patients included 239 with negative pelvic lymph nodes and 53 with positive lymph nodes, who were incorporated into the negative group and the positive group, respectively. Multivariate and univariate analyses of the risk factors of lymph node metastasis and prognosis were performed for both groups.Results:The univariate and multivariate analyses showed that the lesion ≥4 cm, deep interstitial invasion, and the number of risk factors were independent factors influencing pelvic lymph node metastasis ( χ2 = 7.11, 9.05, 90.08, P < 0.05). There was no statistically significant difference in the 3- and 5-year OS ( P>0.05) between both groups. The 3-year disease-free survival (DFS) and 5-year DFS of the negative group were 87.6% and 84.5%, respectively, and those of the positive group were 72.5% and 69.3%, respectively ( χ2=8.59, P=0.003). Regarding failure modes, distant metastasis mainly occurred in the positive group, while local recurrence was dominant in the negative group ( χ2=9.40, P<0.05). The univariate analysis of the DFS in 53 patients with postoperative positive lymph nodes showed that deep interstitial invasion affected DFS, with statistically significant differences ( χ2 = 7.25, P < 0.05). The result of the multivariate analysis showed that the lesion size >4 cm, positive residual, and deep interstitial invasion significantly influenced the DFS ( χ2 = 4.37, 4.69, 4.39, P < 0.05). Conclusions:The lesion size, deep interstitial invasion, and risk factor number were independent factors influencing lymph node metastasis, and the DFS after radiotherapy of patients with pelvic lymph node metastasis was significantly lower than the patients with negative lymph nodes. The main recurrence type of patients with lymph node metastasis was distant metastasis. Moreover, the deep interstitial invasion was independent factor affecting the DFS of early-stage cervical cancer patients with postoperative positive lymph nodes.
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Objective To study the protective effect of active peptide GRGDS on rat nerve cells (PC12 cells) in oxygen glucose deprivation (OGD) injury model and explore its mechanism of action. Methods PC12 cells were divided into control group, ODG group, and active peptide GRGDS treatment group. The injury model was established by simulating in vitro cerebral ischemia by oxygen and sugar deprivation. MTT and flow cytometry were used to detect apoptosis after oxygen-glucose deprivation. ELISA method was used to detect the changes of inflammatory factors TNF-α and IL-1β in PC12 cell supernatant after oxygen-glucose deprivation. Western blot was used to detect the expression of apoptosis pathway-related proteins. Results The results of MTT and flow cytometry showed that the active peptide GRGDS significantly reduced the apoptosis of PC12 cells after oxygen glucose deprivation (P<0.05). ELISA test results showed that the active peptide GRGDS significantly reduced the content of TNF-α and IL-1β in the supernatant of PC12 cells after oxygen-glucose deprivation. (P<0.05). Western blot results showed that the active peptide GRGDS significantly reduced the expression levels of p-JNK, Bax, and cleaved caspase 3 in PC12 cells mediated by oxygen-glucose deprivation injury (P <0.01). Conclusion The active peptide GRGDS has protective effect on PC12 cells damaged by oxygen and glucose deprivation. The mechanism may be related to anti-apoptotic and anti-inflammatory effects.
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Objective:To investigate the clinical application of cervix-uterine internal target volume (ITV) in volume-modulated arc therapy (VMAT) for cervical cancer under different bladder filling conditions, aiming to obtain more accurate planning target volume (PTV).Methods:Sixty-two patients with cervical cancer receiving radiotherapy rather than surgery were selected and randomly divided into the study ( n=31) and control groups ( n=31). In the study group, individualized ITV, PTV and PTV margin were obtained under three bladder filling status by localization CT scan to compare the VMAT. The target area and organs at risk (OARs) within the target area were statistically compared between two groups. The target missing rate in CBCT, adverse events and short-term efficacy of radiotherapy were analyzed in two groups. Results:There was no significant difference in the volume of target area and OARs in the target area between two groups (both P>0.05). In the study group, the target missing rate and target missing volume were significantly lower compared with those in the control group (both P<0.05). There was no significant difference in the incidence of acute radiation-induced adverse events between two groups ( P>0.05). The 1-, 2-year overall survival and progress-free survival did not significantly differ between two groups (all P>0.05). One patient in the study group had uterine recurrence and 2 cases in the control group. Conclusion:Application of individualized cervix-uterine ITV and PTV in definitive VMAT under different bladder filling conditions can improve the accuracy of target area contouring and improve the local control rate in cervical cancer patients.
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Objective:To investigate the role of miR-483-5p in adrenocortical cancer (ACC) and its possible mechanism.Methods:Quantitative real-time polymerase chain reaction was conducted to estimate the expression of miR-483-5p and CDK15 in ACC tissues and cell lines. The effects of miR-483-5p on proliferation were determined in vitro using CCK-8 proliferation assays, the changes of invasion of ACC cells were examined by Transwell. The molecular mechanism underlying the relevance between miR-483-5p and CDK15 was confirmed by luciferase assay and rescue assays.Results:We found a relatively higher miR-483-5p (2.36±1.02 vs 1.09±0.43) and lower CDK15 (0.57±0.26 vs 1.06±0.32) expression in ACC specimens and cell lines. CDK15 was verified as a direct target of miR-483-5p by luciferase assay. over-expression of miR-483-5p promoted proliferation (24 h: 0.26±0.03 vs 0.23±0.04, 48 h: 0.56±0.05 vs 0.41±0.03, 72 h: 0.73±0.04 vs 0.59±0.03) and invasion (95.78±4.66 vs 23.89±2.52) by down-regulating CDK15 expression.Conclusion:miR-483-5p plays a tumorigenesis role in ACC progression by down-regulating CDK15 expression, which may lead to a novel insight to the potential biomarker and novel therapeutic strategies for ACC.
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Objective To summarize and analyze the prevention and control model of colorectal cancer in Nanshan District, Shenzhen, and to provide references for other regions. Methods Based on the three-level prevention and control network in Nanshan District, top specialists from across the country and abroad were brought in serving as a technical support team to explore scientific and feasible early screening techniques and management models for colorectal cancer. Results Through a series of measures, such as government leadership, departmental cooperation, closed-loop management of the prevention and control services, health education and so on, the residents' participation increased and health literacy was also improved. More than 20,000 residents participated in the risk assessment of colorectal cancer, and the positive detection rate was nearly 50% in people at a high risk. Conclusion The public health institution-community-hospital three-level prevention and control network was conducive to improving the compliance of residents with cancer screening, early detection and early intervention to reduce cancer morbidity and mortality.
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Objective@#To investigate the impact of bladder filling status on the movement of uterine and on the volume of organs at risk(OAR), so as to provide theoretical basis for individualized internal target volume (ITV) and planning target volume (PTV).@*Methods@#Simulation CT images for a total of 27 patients with locally advanced cervical cancer were acquired with empty bladder, 1.0 h after bladder filling, 1.5 h after bladder filling, respectively. The volumes of uterine corpus, cervix, OARs, CTV and PTV 1.0 h after bladder filling were delineated. The impact of bladder filling status on the displacements of cervix and corpus, volumetric changes of rectum, small intestine, and the volumes of small intestine, bladder and rectum within PTV were analyzed. The correlation between bladder filling status and uterine displacement and volume of OAR was also investigated, as well as the volume of cervix and corpus in the PTV with empty bladder and 1.5 h after bladder filling.@*Results@#Bladder filling status is different for individual cases. The maximum movement range of cervix and uterine body with bladder filling state was 0-3.04 cm and 0-4.31 cm respectively. The anterior displacements of corpus (F=7.818, P<0.05), the volumetric changes of blander, as well the volume of bladder and small intestine in the PTV (F=46.197, 44.609, 29.546, P<0.05) were significantly different between different bladder filling status. The bladder filling status was correlated with the displacements of the anterior of corpus, volumetric changes of small intestine, and the volumes of bladder and small intestine within the PTV (r=-0.232, -0.298, 0.915, -0.336, P<0.05). The volumes of cervix and corpus out of the PTV were significantly different between the empty bladder and 1.5 h after bladder filling (t=-1.326, -1.559, P<0.05).@*Conclusions@#Bladder filling status was different for individual patients. The displacements of the anterior of the corpus were significantly affected by the bladder filling status. Increasing the anterior margin of PTV was recommended. The consistency of bladder filling status was critical for the precise cervical cancer IMRT.
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Objective To investigate the impact of bladder filling status on the movement of uterine and on the volume of organs at risk(OAR), so as to provide theoretical basis for individualized internal target volume ( ITV) and planning target volume ( PTV) . Methods Simulation CT images for a total of 27 patients with locally advanced cervical cancer were acquired with empty bladder, 1. 0 h after bladder filling, 1. 5 h after bladder filling, respectively. The volumes of uterine corpus, cervix, OARs, CTV and PTV 1. 0 h after bladder filling were delineated. The impact of bladder filling status on the displacements of cervix and corpus, volumetric changes of rectum, small intestine, and the volumes of small intestine, bladder and rectum within PTV were analyzed. The correlation between bladder filling status and uterine displacement and volume of OAR was also investigated, as well as the volume of cervix and corpus in the PTV with empty bladder and 1. 5 h after bladder filling. Results Bladder filling status is different for individual cases. The maximum movement range of cervix and uterine body with bladder filling state was 0-3.04 cm and 0-4.31 cm respectively. The anterior displacements of corpus (F=7. 818, P<0. 05) , the volumetric changes of blander, as well the volume of bladder and small intestine in the PTV ( F=46. 197, 44. 609, 29. 546, P<0. 05 ) were significantly different between different bladder filling status. The bladder filling status was correlated with the displacements of the anterior of corpus, volumetric changes of small intestine, and the volumes of bladder and small intestine within the PTV ( r=-0. 232,-0. 298, 0. 915, -0. 336, P<0. 05) . The volumes of cervix and corpus out of the PTV were significantly different between the empty bladder and 1. 5 h after bladder filling ( t=-1. 326, -1. 559, P<0. 05 ) . Conclusions Bladder filling status was different for individual patients. The displacements of the anterior of the corpus were significantly affected by the bladder filling status. Increasing the anterior margin of PTV was recommended. The consistency of bladder filling status was critical for the precise cervical cancer IMRT.
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Objective To investigate the therapeutic influence of short segment fixation with navigated percutaneous pedicle screws in the treatment of thoracolumbar compression fracture and whether use of pedicle screw fixation affects the treatment efficacy.Methods A retrospective case control study was conducted on the clinical data of 38 patients with thoracolumbar compression fracture admitted between January 2014 and October 2016.There were 27 males and 11 females,with an average age of 35.9 years (range,21-59 years).There were 14 cases with injured segment at T12,14 cases at L1,and 10 cases at L2,all of which were classified as Type A according to AO typing.The patients were divided into screw group (n =22) and non-screw group (n =16).The pedicle screws were placed into the upper and lower adjacent segments of the fracture vertebra or the fracture vertebra by percutaneous fluoroscopyguided navigation.The operation time,intraoperative blood loss,Visual analogue scale (VAS) before and after operation,anterior vertebral body height,Cobb angle,and complications in two groups were recorded.Results All patients were followed up for 12-30 months,average 15.2 months.No significant differences were found between the screw group and the non-screw group with respect to operation time [(44.7 ±12.6)minutes vs.(39.3 ±11.9)minutes] and blood loss [(56.8 ±13)ml vs.(48.4 ± 11.0)ml](P ＞0.05).In the screw group,preoperative VAS was (6.7 ± 1.5) points,and VAS at postoperative 1 week,six months and 1 year were (2.3 ± 0.9) points,(1.1 ± 0.3) points and (0.9 ± 0.5) points,respectively (P ＜ 0.01).In the non-screw group,preoperative VAS was (6.1 ± 1.7) points,and VAS at postoperative 1 week,six months and 1 year were (2.1 ± 0.8) points,(1.2-± 0.6) points and (1.0-±0.6) points,respectively (P ＜0.01).No significant difference was found on VAS between the two groups (P ＞ 0.05).In the screw group,anterior vertebral body height was (59.5 ±-1 3.6) ％ preoperatively,and anterior vertebral body height at postoperative 1 week,six months and 1 year was (90.6±7.7)％,(89.3 ±8.3)％ and (88.4 ±7.9)％ (P＜0.01).In the non-screw group,preoperative anterior vertebral body height was (60.1 ± 12.5)％,and anterior vertebral body height at postoperative 1 week,six months and 1 year was (89.3 ±8.9)％,(88.4 ±9.1)％ and (86.5 ±8.7)％ (P ＜0.01).No significant difference was found on anterior vertebral body height between the two groups (P＞0.05).In the screw group,preoperative Cobb angle was (15.6 ±4.1)°,and Cobb angle at postoperative 1 week,six months and 1 year was (3.0 ±1.8)°,(5.0 ±1.9) ° and (5.9 ±1.8)°,respectively (P ＜ 0.01).In the non-screw group,preoperative Cobb angle was (16.2 ± 5.1) °,and Cobb angle at postoperative 1 week,six months and 1 year was (3.1 ±1.4)°,(5.2 ±2.3)° and (5.6 ± 2.1) °,respectively (P ＜ 0.01).No significant difference was found on Cobb angle between the two groups (P ＞ 0.05).No spinal cord injuries because of pedicle screws were observed after operation in both groups.Conclusions For thoracolumbar compression fracture,short segment fixation with navigated percutaneous pedicle screws has the advantages of shorter operation time,less bleeding,better postoperative pain relief,and fewer complications.In addition,the postoperative VAS,anterior vertebral body height and sagittal Cobb angle are improved significantly.However,use of pedicle screw fixation in fracture vertebra does not have significant influence on the treatment efficacy.
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This paper was aimed to study traditional Chinese medicine (TCM) syndrome distribution characteristics of HIV infection in Xinjiang region based on the Clinical Medical Research Information Sharing System (CMRISS).CMRISS was used to establish a database (data were from 1151 hospital electronic medical records from May 2011 to March 2012).Oracle and ETL software were used in the data processing.Visualization analysis was used in the data mining.The results showed that syndrome distribution of HIV infection in Xinjiang region was concentrated in four categories with the blockade of dampness due to qi deficiency,deficiency of both qi and yin,yin deficiency of liver and kidney,and the stagnation of liver qi.The syndrome of blockade of dampness due to qi deficiency was relatively concentrated in the range of 20-30 years old,with the majority of female population.The proportion of sexually transmitted infection was more.The syndrome of deficiency of both qi and yin was relatively concentrated in the age range of 30-40 years old,with the majority of male population.The large proportion of infection was due to intravenous drug use.Among different TCM syndromes,Uyghur population occupied relatively large part.Stratified analysis on disease course due to the change of TCM syndrome according to 1 year,3 years and 5 years revealed the disease development rule from excess syndrome,deficiency combined with excess,to deficiency syndrome.It was concluded that the application of CMRISS was able to process a large amount of clinical data.The data mining results can be used to guide clinical practice.It provided a better platform for the scientific research of TCM clinical practice.
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ObjectiveTo evaluate the vitro anti-HIV-1 activity ofTaiqi Peiyuan Granules.MethodsMTT was used to detect cytotoxicity ofTaiqi Peiyuan Granules; cytopathy method was used to detect the inhibitory activity of Taiqi Peiyuan Granules on acute infection of HIV-1; HIV-1 p24 antigen ELISA detection was used to detect inhibitory activity ofTaiqi Peiyuan Granules for virus replication of HIV-1 acute infection cells, and count the medical therapeutic indexes.ResultsCC50ofTaiqi Peiyuan Granules in cytotoxicity test was 3.761±0.370 mg/mL; the EC50 of inhibition syncytial test was 0.454 5±0.204 6 mg/mL; the therapeutic index was between 5.84 and 12.97; p24 ofTaiqi Peiyuan Granules in inhibition experiments was 0.56±0.27 mg/mL, and the therapeutic index was between 5.30 and 8.74.ConclusionAnti-HIV-1 activity ofTaiqi Peiyuan Granules is relatively weak.
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Objective To understand the factors related with child restraint system (CRS) use,and provide evidence for the development of appropriate intervention measures to promote the use of CRS for the protection of child passengers' safety.Methods Self-administrated questionnaire survey was conducted among 0-6 years old children' s parents who owned private cars selected through stratified cluster random sampling in Shanghai and Shenzhen to collect date about CRS use and related factors.Group discussion was conducted among some of the parents randomly selected to further understand the reasons for using or not using CRS.Results Of 7 528 parents surveyed,39.23 ％ (2 820/7 189) reported to have CRS and 17.14％(1 232/7 189) reported consistent use of CRS.Multivariate analysis indicated that young age of children,high level of education of parents,good family economic status,awareness of importance of children's safety were the positive factors for CRS use.The frequency and distance of children' car taking and the seatbelt use of drivers significantly influenced the CRS use.The main reasons for not purchasing CRS included limited car space (53.33％,2 329/4 367),low frequency of children car taking (48.55％,2 120/4 367),difficulty in installation (42.25％,1 845/4 367),high cost (38.58％,1 685/4 367),and unreliable quality (31.03％,1 355/4 367).The main reasons for not using CRS included children' s refusal (67.36％,293/435),short travel distance (53.79％,234/435),difficulty in installation or use (53.10％,231/435),limited car space (32.41％,141/435),and unnecessary (25.75％,112/435).Conclusions Parents have gaps and misunderstandings in using CRSs to protect child passengers safety.There are demands of technical guiding service in use of CRS.Integrated intervention measures should be implemented targeting at the identified barriers and needs in CRS use to promote child passenger safety,which include strengthening the propaganda and education,promoting the legislation and law enforcement,strengthening market supervision,establishing CRS related services site,exploring the rental market,etc.
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This study was aimed to observe the clinical efficacy of Highly Active Antiretroviral Therapy (HAART) combined withPing-Ai(PA) Mixture in the treatment of Human Immunodeficiency Virus / Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with non-infectious skin lesions, in order to study the advantages of integrative medicine in AIDS prevention and treatment. A total of 9 cases, who met the inclusion criteria of AIDS-associated non-infectious skin lesion with the pattern ofqi-yin deficiency and internal obstruction of phlegm-stasis, were selected. The combination of HAART and number 1 and 4 prescription of PA Mixture were used in the treatment for 3 months. The observations were made on changes of clinical symptoms, body signs, Karovsky integral and CD4+ T cells of patients before and after treatment. Stata 12.0 was used in the statistical analysis. The results showed that after integrative therapy, the single integral of clinical symptoms and body signs, such as skin spot papula, subcutaneous nodules, skin itching, fatigue was obviously reduced compared to that of the pretreatment. The total score decreased than that of the pretreatment. The patients’ quality of life (QOL) significantly increased. The Karovsky integral increased compared with that of pretreatment. The CD4+ T cells values were higher than that of the pretreatment. It was concluded that the integrative therapy had a certain effect in the treatment of AIDS-associated non-infectious skin lesions, which can improve the clinical symptoms, body signs, obviously increase the patients’ QOL and immune function.
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This study was aimed to observe the clinical effect of Pingai Mixture intervention on HIV/AIDS patients in order to clarify the characteristics of Pingai Mixture for AIDS treatment. A total of 41 HIV/AIDS patients who met the inclusion criteria were selected. Pingai Mixture was applied in the treatment. Three months was one treatment course. The treatment was given for two courses. Clinical symptoms, physical signs and changes in CD4+ lymphocytes of HIV/AIDS patients before and after treatment were observed. The SPSS software was used for statistical analysis. The results showed that the posttreatment total score (primary, secondary symptoms and the main physical sign cred-its) was significantly reduced compared with pretreatment (P < 0.01). The posttreatment spontaneous perspiration, night sweats, hair loss and other symptoms of a separate score was significantly reduced compared with pretreatment (P< 0.05). There were no significant changes on other symptoms or physical sign indicators. There was no significant difference in the Karnovsky score after 6-month treatment compared with pretreatment. It was concluded that Pingai Mixture is effective in AIDS treatment. It can improve the clinical symptoms and the administration is safe.
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This article was aimed to introduce the research status on HIV/AIDS TCM syndrome and Chinese medicine treatment in China . It also elaborates on the results of HIV/AIDS TCM syndrome and Chinese medicine treatment in X inj iang region . A summary of the existing problems in the research of HIV/AIDS with TCM preven-tion and treatment in China was given and suggestions were put forward .
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This study was aimed to observe the clinical effect of Ping-Ai GranulesⅠin the treatment of HIV/AIDS . A total of 107 AIDS cases were divided into Ping-Ai GranulesⅠgroup and Ping-Ai GranulesⅠunited with HAART group. The results showed that there was significant difference between Ping-Ai GranulesⅠgroup and Ping-Ai GranulesⅠunited with HAART group in aspects of symptoms, physical signs, Karnovsky Score and changes of CD4+ T lymphocyte . It was concluded that Ping-Ai GranulesⅠhad the function of improving patient's symptom and increasing patient's immunity and living quality .
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This study was aimed to summarize the medicine operation mode of fighting against AIDS in Xinjiang region in order to play its advantages and find its insufficiencies. The summarization had been made on aspects of resource integration , mode creation , highlighting research priorities and mechanism improvement . Advantages of various aspects had been pointed out and existing problems had been proposed. It was concluded that AIDS study by the National Clinical Research Base of Traditional Chinese Medicine in X inj iang still needs to share its re-source, innovate its network system construction model, operate its standard diagnosis and treatment, mechanism and system, and further strengthen its personnel training.
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To study the characteristics of traditional Chinese medicine (TCM) syndrome factors of patients from different areas of China with human immunodeficiency virus (HIV) infection or acquired immunodeficiency syndrome (AIDS).
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Objective To evaluate the ability of 3-AB to sensitize the human esophageal carcinoma cell strain (CaEs-17) to radiation in v/tro and its mechanisms. Methods CaEs-17 cells were treated with 3-AB at 0, 2.5, 7.5 mmol/L and given irradiation O, 3, 6, 9, 12 Gy. 3-AB concentration in each group was made dose-survival curve using multi-target single-hit maiths model by clonogenie assay. MTT assay was performed to observe the survival of irradiated cells.comet assay and metaphase chromosome analysis were used to measure the DNA damage degree and chromosome aberration of CaEs-17 cell after 3-AB treatment and irradiation. Results Cell survival experiments showed SER of 1.21, 1.52 for 2.5 mmol/L, 7.5 mmol/L 3-AB respectively using multi-target single-hit maths model. The survival fraction of irradiated CaEs-17 cell was decreased after 3-AB treatment. DNA damage and the chromatid breakage number of irradiated CaEs-17 cells were increased after 3-AB treatment. Conclusions 3-AB, a PARP inhibitor, can enhance the radiosensitivity of human esophageal carcinoma cell strain (CaEs-17). DNA damage repair inhibition by 3-AB might be one of the mechanisms.
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<p><b>OBJECTIVE</b>To investigate the alkaloids in the roots of Dactylicapnos scanden (D. Don) Hutch.</p><p><b>METHOD</b>The compounds were isolated by various column chromatographic methods. The structures were elucidated by spectroscopic analysis.</p><p><b>RESULT</b>Eight compounds were isolated and identified as d-isocorydine (1), protopine (2), d-magnoflorine (3), d-isocorydine-beta-N-oxide (4), d-corydine-alpha-N-oxide (5), d-corydine-beta-N-oxide (6), 6S, 6aS-N-methyllaurotetanine-alpha-N-oxide (7), and 6R, 6aS-N-methyllaurotetanine-beta-N-oxide (8).</p><p><b>CONCLUSION</b>Compounds 4-8 were isolated from this species and the genus Dactylicapnos for the first time.</p>